miRNAsong

Search for specific microRNA and design a sponge:

There are 35828 MicroRNA sequences from 219 of species in our database.

Select species (?)Select species to search for specific microRNA.:	MicroRNA: Name (?)Name e.g. miR-424 Accession Number (?)Accession Number e.g. MIMAT0001341:

Design a sponge to single microRNA using microRNA sequence:

MicroRNA Sequence (?)15-30 nucleotides 5'->3' direction:	Make a bulge (?)microRNA sponges having a bulge are reported to be more efficient.:	Number of miRNA binding sites in a sequence (?)Number of sites binding selected microRNA in generated sponge:	Spacer sequence between sponges (?)MicroRNA binding sites are separated with this "spacer" sequence. Enter 4-6 nucleotides (RNA):
5' -- 3'			5'--3'
Sponge testing options:
Energy cut-off (?)Energy cut-off per interaction.:	Test generated sponge for potential off-targets in (?)Select species for sponge testing.:	Seed region features (?)Force miRNAsong to predict miRNAs with perfect match at seed region. Set the nature of seed region.:
		Canonical: 6-mer seed (2-7) Canonical: 7-mer seed (2-8) Canonical: offset 6-mer seed (3-8) All interactions including non-canonical

Test sponge sequence:

Sponge sequence (?)5'->3' DNA sense strand. 30-150 nucleotides:	Test sponge for MicroRNAs in (?)Select species for sponge testing.:	Energy cut-off (?)Energy cut-off per interaction.:	Seed region features (?)Force miRNAsong to predict miRNAs with perfect match at seed region. Set the nature of seed region.:
			Canonical: 6-mer seed (2-7) Canonical: 7-mer seed (2-8) Canonical: offset 6-mer seed (3-8) All interactions including non-canonical

Design and test sponge for multiple microRNAs or microRNA family:

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